research ultrasound system vantage 256 system  (Verasonics Inc)

Journal: Advanced Science

Article Title: Label‐Free Dual‐Modal Photoacoustic/Ultrasound Localization Imaging for Studying Acute Kidney Injury

doi: 10.1002/advs.202414306

Figure Lengend Snippet: Schematic representation of label‐free 3D PAUL imaging. a) Illustration of kidney imaging with mechanical‐scanning based dual modal photoacoustic (PA) and ultrasound (US) imaging. The central frequency of the diagnostic transducer is 15 MHz. The laser fluence is under 20 mJ cm −2 . b) During data acquisition, ultrafast ultrasound imaging and multi‐wavelength PA imaging are recorded alternatively at each position. For each position, US images with 5 s acquisition were collected at frame rate of 500 Hz. Multiwavelength PA images (MvPA, 750 and 850 nm) with 1 s acquisition were collected at frame rate of 10 Hz. c) Schematic of PA and label‐free UL acquisition. d) Flowchart of dual modal photoacoustic and ultrasound imaging postprocessing. Clutter filtering was applied to extract blood signal and label‐free super‐resolution ultrasound image was generated by localizing erythrocytes within blood signals. Functional photoacoustic information was extracted by spectrum unmixing of MwPA images. e) Mechanical scanning was conduct to collect 3D kidney structural and physiological information. f) One representative whole kidney microvasculature and oxygenation map of a mouse. g) Quantitative analysis of kidney volume, kidney vasculature and kidney oxygenation from 3D dual modal photoacoustic and ultrasound image. ILAV, interlobular artery‐vein; RAV, renal artery‐vein; IAV, interlobar artery‐vein; and AAV, arcuate artery‐vein.

Article Snippet: The dual‐modal PAUL system consisted of a Verasonics ultrasound imaging research system (Vantage 256, Verasonics Inc., Kirkland, WA, USA), a 15 MHz customized linear array Vermon), and a wavelength tunable (690–950 nm) OPO laser source with 7‐ns‐pulse and 10 Hz pulse repletion rate (Phocus Essential, Opotek, Inc., Carlsbad, CA, USA).

Techniques: Imaging, Diagnostic Assay, Generated, Functional Assay

Journal: Advanced Science

Article Title: Label‐Free Dual‐Modal Photoacoustic/Ultrasound Localization Imaging for Studying Acute Kidney Injury

doi: 10.1002/advs.202414306

Figure Lengend Snippet: Characterization of label‐free PAUL imaging. a) An ultrasound B‐mode image of a mouse kidney. The boundary of the kidney is indicated by the white dash curve. b) A power Doppler image of a mouse kidney. c) A label‐free super‐resolution ultrasound image of a mouse kidney. (d) Zoom‐in vasculature region from (b) and (c). e) 1D vessel profile of the label‐free UL and power Doppler image at line 1 and line 2 indicated in (b) and (c). f) PA images of a mouse kidney collected at 750 and 850 nm, respectively. g) The difference PA image. h) Comparison of PA signal at 750and 850 nm. The error bars are standard deviations (N = 3). Unpaired student t ‐test is used. i) Hemoglobin (Hb) and oxyhemoglobin (HbO2) concentration distribution in a mouse kidney. The information was extracted from multiwavelength PA images (750 and 850 nm) via spectrum unmixing. j) Oxygenation saturation (SO2) map of a mouse kidney. k) Comparison of HbO2 and Hb concentration. The error bars are standard deviations (N = 3). Unpaired student t ‐test is used. **** p < 0.0001.

Article Snippet: The dual‐modal PAUL system consisted of a Verasonics ultrasound imaging research system (Vantage 256, Verasonics Inc., Kirkland, WA, USA), a 15 MHz customized linear array Vermon), and a wavelength tunable (690–950 nm) OPO laser source with 7‐ns‐pulse and 10 Hz pulse repletion rate (Phocus Essential, Opotek, Inc., Carlsbad, CA, USA).

Techniques: Imaging, Comparison, Concentration Assay